Supersedes 1. Description Figure 1. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. M2. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. SoftMax Pro 7. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. The temperature in the plate chamber is ambient and isothermal. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. 40266 Email: drugdiscoverytech@lifetech. Data collection speed is sufficient for running fast cell-based kinetic assays. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Absorbance Plate Reader Comparison Chart. 0. 1. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. was added and absorbance was measured at 450 nm and 650 nm using a plate reader (Molecular Devices, SpectraMax ABS Plus, SoftMax Pro 7. It has dual scanning monochromators that allow for 1 nm increment selection over the wavelength range of 250 - 850 nm. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Data collection speed is sufficient for running fast cell-based kinetic assays. Superior optics Microplate Reader Shelf. Next generation SpectraMax® absorbance microplate readers. Fixed cells stained with fluoresceinconjugated anti-VCAM are identified with the Marker Expression Protocol. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. In the M2, M5 and Plus. SpectraMax i3x. APP Automated. Supersedes 1. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. What plates do you recommend for SpectraMax Plate Readers? Luminescence : Solid white plates for top reads and white plates with clear bottoms for. The opaque walls prevent stray light from being detected from neighbouring. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmFor the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. W×D×H. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. View the Molecular Devices website. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Summary of Contents for Molecular Devices SpectraMax M2. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Services. Plate reader instructions Computer. Data analysis. #0200-7011) Instrument setupSOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 9. Company Molecular Devices, LLC. Learn how Molecular Device's VersaMax Microplate Reader can be combined with the Pyrochrome Assay from Associates of Cape Cod, Inc. With various on-board features and. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. We would like to show you a description here but the site won’t allow us. Absorbance Plate Reader Comparison Chart. These robust readers have been placed in labs from. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Important things to consider when purchasing a multimode microplate reader. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. A preconfigured. Visible absorbance -. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. 0 . Catalog Number i3x. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. S. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices. AXON Patch-Clamp. S. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Molecular Devices SpectraMax 384 Plus Microplate Reader. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Instrument settings can be saved as a protocol file. The adapter is required for optimum performance with standard 96-well and 384-. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader ( Molecular Devices, San Jose, CA, USA) at 30 °C. in steps of 0. visible absorbance, fluorescence. The instrument settings were identical to those used in Example 1 above. Used. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. 2. 0 . 1. 25 to 3. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. +1 800-635-5577. If no sound is heard during initialization, contact technical support. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Lab automation for high-throughput plate-based assays. Remove the lamp screw (on the right side of the lamp, as shown in the diagram) then gently pull the connecting wires to remove the lamp from the body of the instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. begin reading microplates with seven mouse clicks. The plate reader optics are tunable for absorbance. and the assembly was incubated for 5 minutes in the dark prior to reading the plate. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. 0A-Sunnyvale, CA-Made in USAMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The computer controlling the plate reader runs Windows XP. VIS absorbance detection. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. PURPOSE. Microplate Readers. Description 1. TheUnparalleled performance on a personalized platform. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 2. Also see: SoftMax® Pro Microplate Software User Manual Download Page. 1 sec. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. PathCheck sensor corrects optical pathlength & pipetting variation. IMAP assays are based on binding of. 001 EU/mL) was added in order to test the sensitivity of the SpectraMax i3 reader. 0A Sunnyvale, CA Made in USAFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Molecular Devices' microplate readers. . BMG Labtech - THERMOstar. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax:. 1. The plate reader optics are tunable for absorbance,. We would like to show you a description here but the site won’t allow us. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. The plate reader optics are tunable for absorbance, fluorescence intensity,. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. • 0. 0 EU/mL, but the additional standard concentration (0. Firmly grasp the snap connector beside the snap, then push the snap down. ~ Bio-Tek ELx808 Microplate Reader $2,795. PURPOSE. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The chamber contains springs that position the cuvette in the proper alignment for a read. Read one sample or up to 384 in a. 9000-0756. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. The concentration of total free thiols in the protein. Select the Driver tab and click Update Driver. plate type, read area and number of images per well, plus specify positive/negative wells. Turn the reader on and secure the USB cable. With optimized reagents, option to operate the readers. absorbance plate readers. the iD5 reader’s filter system. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. the use of a reader calibrated with primary NIST and NMI standards. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Details. This can increase throughput and efficiency, thus saving time and space in the lab. Cells in each. Perform the following steps to install the drivers: Start > Control Panel > System. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Fig 2: Enzymatic response of synthetic compounds. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 9000-0756. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPrimary considerations when choosing a microplate reader are throughput and flexibility. Built-in near-field communication (NFC) reader enables you to pull up your custom protocols and results with a single tap, saving you precious time. 190. Set up the instrument and software. HTRF-compatible SpectraMax Multi-Mode Readers. Molecular Devices makes. FlexStation™ II Systems provide integrated fluid transfer capability from a source plate or reservoir to an assay plate with concurrent kinetic fluorometric analysis of reactions. In an analogous manner, the same sample names were assigned to a CuvetteSet section. 0) OD (0. In addition, users can define individual reagents and concentrations to be delivered to each well. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Integrated stacker option. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. coli stock with an OD 600 value of 0. Absorbance, fluorescence, and luminescence detection with upgradeable high-performance capabilities. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. EMax. The Back Panel Figure 1. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Devices’ StakMax® Microplate Stacker in a matter of minutes and . Molecular DevicesThe Marker Expression Protocol is used to identify cell areas labeled with the fluorescein-conjugated anti-VCAM antibody. Explore our fully-integrated,. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Unplug the power cable from the back of the instrument. Step 2: Launch SoftMax Pro Software. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesPlate reader instructions Computer. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. 9000-0756. Plate reader. integration time per well, depending on firmware version. This offer includes SpectraMax i3x, SpectraMax. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on. Cell line development teams will realize savings on time and resources,Some plate readers handle label-free cell-based assays, such as PerkinElmer’s EnSpire® multimode reader, which in addition to absorbance, luminescence, fluorescence and AlphaLISA also supports Corning’s Epic® technology. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Cell viability는 세포막 Integrity 또는. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Open the top cover of the instrument. 3860 N First Street San Jose,CA 95134 USA +1 408. Instrument settings can be saved as a protocol file. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 72 MB. Reader Documentation Version No. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 0 × 107) RLU 0. Recertification service. Consider non binding surface type plate (Greiner and Corning are 2 manufacturers)Molecular Devices microplate readers. Gregory et al. (Molecular Devices cat. 0%, raw data units = counts/sec. 더 많은. Your applications, your modes, your choice. Number of Citations*: 8350. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. (7) Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The sample is typically placed in a cuvette through which light is sent horizontally. When connecting for the first time to either the SpectraMax L reader or StakMax system. The direct transfer capability reduces We would like to show you a description here but the site won’t allow us. Step 1: Turn on your microplate reader. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Gemini EM is a microplate spectrofluorometer from Molecular Devices. ) Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Contact our support team with your questions regarding Molecular Devices products and applications. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Step 4: Incubate at room temperature for 1 hour, protected from light. If no sound is heard during initialization, contact technical support. ORDER. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). It is more inert and reduces compound interference. This document is provided to customers who have purchased Molecular Devices, Inc. 1-10 sec. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. W×D×H. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Description: ScanLater* Western Blot Detection Cartridge, enables the first western blot detection capability to a microplate reader, Time-resolved fluorescence detection, Xenon flash lamp excitation source. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 2. Tests every subsystem of the reader . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Versa. Retrieving Each. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 6 % of reading 4. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Flexibility to read a variety of plate formats. SoftMax® Pro 7. For SpectraMax ABS Plus reader, r 2 = 0. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. SpectraMax M5. We therefore gathered salient. 1 sec. Molecular Devices Software. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. please click here. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Standard features include a cuvette port,. 1. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Examples include ELISAs for quantitation of cytokines and protein concentration determination using the Bradford assay. Step 3: Click “Settings” and a screen will appear (Figure 1). . The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The content of this site is published by the site. Plate pre-read measurements were made in a Costar UV plate. Read height should be optimized for Vmax® plate reader specifications. Every step is optimized to simplify analysis and reporting to support our microplate readers. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. , June 1, 2004 - Molecular Devices Corporation (Nasdaq: MDCC), today announced the introduction of FLIPRTETRA the first system that enables researchers to significantly increase throughput of cell-based assays by measuring 1536 kinetic measurements simultaneously. The FLIPRTETRA, which debuts today at. Plate Formats: 6 to 384-well microplates. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). One plate carrier adapter is provided with the instrument. Superior optical system includes a xenon flash lamp and features. Cells in each image are identified by SoftMax Pro Software and cell-by-cellOne plate carrier adapter is provided with the instrument. US HEADQUARTERS. Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please click on the links below to. After reading the ELISA plate on both readers, each standard curve was nearly identical. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. Avoid losing high signal. Ensure data accuracy of your reader. Microplate Reader Shelf. The homogeneous Neurotransmitter Transporter Uptake Assay Kit from Molecular Devices Corporation provides a fast, simple and reliable fluorescence-based. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Imaging with the MiniMax 300 Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Lower variance: Normalize your plate reader assay data to the number of cells in each well. Item SpectraMax i3x Multi-Mode Microplate Reader. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 0) OD (500 to 1100) RFU (5. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Introduction. Step 2: Launch SoftMax Pro Software. Monday to Friday 8am - 5pm GMT. 5. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. A control well was set up to verify the accuracy of the standard curve. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. However, when a specific probe is not available, or it causes off-target effects on the. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. In . For customers outside the U. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 00. Category 2 and 3 microplate readers use gradient heating as well. Imaging System Giles Scientific Biomic V3 Automatic Plate. Flexibility to read a variety of plate formats. Clone Screening. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Check software compatibility with Windows 10 and locate your software installer. Molecular Devices. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. 0200-6263. 5. 5: Schematic of. The assay can be performed on any bottom-read mode fluorescence. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. View SoftMax Pro GxP. Figure 9. Page 1 of 8 . SpectraMax Absorbance, Fluorescence. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. ®100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Molecular Devices' microplate readers. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 5032018C 8 Chapter1:Introduction TheEMax® PlusMicroplateReaderisafilter-basedabsorbancemicroplatereaderwitheight standardfiltersforawiderangeofapplications. SpectraTest® Validation Package User Manual Download Page. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Page 1 of 8 . The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. With optimized reagents, option to operate the. If it looks fine, then proceed. Our microplate readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Sale price $18,499. Select Start>Control panel>Device Manager. Select Start>Control panel>Device Manager. Also refer to the Multimode Reader Comparison Chart Features. 1 up to 3600 sec. Microplate Readers. Product Detail. Products : General, FilterMax F5 reader. the standard plate reader applications, protecting your initial investment. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. View SoftMax Pro GxP. Perkin Elmer Victor X2 Multimode Microplate Plate Reader 2030-0020 w/ PC. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below.